|
KMID : 0545120040140030599
|
|
Journal of Microbiology and Biotechnology
2004 Volume.14 No. 3 p.599 ~ p.606
|
|
|
Molecular Differentiation of Bacillus spp. Antagonistic Against Phytopathogenic Fungi Causing Damping-off Disease
|
|
Cho MJ
Kim YK/Ka JO
|
|
|
Abstract
|
|
|
|
Gram-positive antagonistic bacilli were isolated from agricultural soils for possible use in biocontrol of plant pathogenic fungi Fusarium oxysporum Rhizoctonia solani and/or Pythium ultimum. Among the 65 antagonistic Grampositive soil isolates 22 strains were identified as Bacillus species by 16S rDNA sequence analyses. Four strains including DF14 especially exhibited multiple antagonistic properties against the three damping-off fungi. Genotypic properties of the Bacillus isolates were characterized by rapid molecular fingerprinting methods using repetitive extragenic palindromic-PCR (REP-PCR) ribosomal intergenic spacerlength polymorphisms (RIS-LP) 16S rDNA PCR-restriction fragment length polymorphisms (PCR-RFLP) and strainspecific PCR assays. The results indicated that the REP-PCR method was more valuable than the RIS-LP and 16S rDNA PCR-RFLP analyses as a rapid and reliable approach for bacilli typing and identification. The use of strain-specific primers designed based on 16S rDNA sequence comparisons enabled it to be possible to selectively detect a strain DF14 which is being used as a biocontrol agent against damping-off fungi.
|
|
|
KEYWORD
|
|
|
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
  
|